- Basic science and research
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Two-photon intravital imaging of leukocyte migration during inflammation in the respiratory system
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Young Min Kim, Soi Jeong, Young Ho Choe, Young-Min Hyun
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Acute Crit Care. 2019;34(2):101-107. Published online May 31, 2019
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DOI: https://doi.org/10.4266/acc.2019.00542
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 Abstract
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- Two-photon intravital imaging is a powerful method by which researchers are able to directly observe biological phenomena in live organisms. Researchers in various biomedical research fields have applied two-photon imaging to a variety of target organs by utilizing this technology’s ability to penetrate to significant depths with minimal phototoxicity. The mouse respiratory system in inflammation models is a good example, as two-photon intravital imaging can provide insights as to how the immune system is activated in response to inflammation within the respiratory system. Inflammation models can be generated via influenza viral, bacterial, or lipopolysaccharide injection. To exteriorize the lungs or trachea, thoracotomy or tracheotomy is performed, respectively; the appropriate combination of inflammation induction and organ exposure is selected depending on the study purpose. On the other hand, visualizing the movement of leukocytes is also an important component; to this end, immune cell populations of interest are either labeled via the genetic attachment of fluorescent proteins or stained with antibodies or dyes. With the proper selection of methods at each step, twophoton intravital imaging can yield visual evidence regarding immune responses to inflammation.
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Citations
Citations to this article as recorded by  - Live-Cell Imaging Quantifies Changes in Function and Metabolic NADH Autofluorescence During Macrophage-Mediated Phagocytosis of Tumor Cells
Shelby N. Bess, Matthew J. Igoe, Timothy J. Muldoon
Immunological Investigations.2024; 53(2): 210. CrossRef - Integration of immune cells in organs-on-chips: a tutorial
Lisette Van Os, Britta Engelhardt, Olivier T. Guenat
Frontiers in Bioengineering and Biotechnology.2023;[Epub] CrossRef - Intravital Imaging of Pulmonary Immune Response in Inflammation and Infection
Nazli Alizadeh-Tabrizi, Stefan Hall, Christian Lehmann
Frontiers in Cell and Developmental Biology.2021;[Epub] CrossRef - Probe-based intravital microscopy: filling the gap between in vivo imaging and tissue sample microscopy in basic research and clinical applications
Katrien Van Dyck, Eliane Vanhoffelen, Jonas Yserbyt, Patrick Van Dijck, Marco Erreni, Sophie Hernot, Greetje Vande Velde
Journal of Physics: Photonics.2021; 3(3): 032003. CrossRef
- Neurosurgery/Hematology
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Fatal Intracranial Hemorrhage in a Patient with Disseminated Intravascular Coagulation associated with Sepsis
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Hyun Jin Baek, Doo Hyuk Lee, Kyu Hyung Han, Young Min Kim, Hyunbeom Kim, Byeongwook Cho, Inkuk Lee, Kanghyun Choi, Hojin Yong, Goohyeon Hong
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Korean J Crit Care Med. 2016;31(2):134-139. Published online May 31, 2016
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DOI: https://doi.org/10.4266/kjccm.2016.31.2.134
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16,631
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Abstract
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- In critically ill patients, disseminated intravascular coagulation (DIC) is a common and fatal hematological disorder. DIC is a physiological response to a variety of underlying stimuli that provoke generalized activation of the hemostatic mechanism and is common in septic patients and those with hematological or non-hematological malignant neoplasms. Bleeding is a common clinical feature, and diffuse or multiple-site mucocutaneous bleeding, such as petechia, ecchymosis and hemorrhage from gastrointestinal tract, is often seen. A 58-year-old male was recently diagnosed with intracranial hemorrhage (ICH) caused by DIC associated with sepsis. Mortality of ICH caused by DIC is very high because the underlying condition cannot be quickly treated. Awareness of the possibility of DIC developing in a critically ill patient and the need for immediate initiation of plasma or platelet replacement therapy are important. To the best of our knowledge, this is the first reported case of intracranial hemorrhage in a Korean patient with DIC associated with sepsis.
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Citations
Citations to this article as recorded by - Abdominal mass in a septic infant: Case of a fatal intraluminal intestinal hematoma
Mana Taweevisit, Paul Scott Thorner
Pediatric Hematology Oncology Journal.2021; 6(3): 139. CrossRef - Perforated Mesenteric Cyst with Sepsis and Neurological Complication in a 9 Month Old Child
Mandal KC, Saha D, Halder P, Chakraborty P, Debnath B, Mukhopadhyay B
Asploro Journal of Pediatrics and Child Health.2020; 2(1): 30. CrossRef
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